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SDS-polyacrylamide gel electrophoresis

См. также в других словарях:

  • SDS–polyacrylamide gel electrophoresis — (SDS PAGE) a type of polyacrylamide gel electrophoresis in which the anionic detergent sodium dodecyl sulfate (SDS) is used to denature the sample proteins into linear monomers, rendering their charge proportional to their length so that… …   Medical dictionary

  • polyacrylamide gel electrophoresis — (PAGE) gel electrophoresis using a polymerized polyacrylamide matrix to separate molecules on the basis of size, charge, or both; usually used to separate proteins or sequence nucleic acids. Gels are usually discontinuous (see disc e.) but may be …   Medical dictionary

  • Gel electrophoresis — electrophoresis electrophoresis n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet… …   The Collaborative International Dictionary of English

  • polyacrylamide gel electrophoresis — (= PAGE) Analytical and separative technique in which molecules, particularly proteins, are separated by their different electrophoretic mobilities in a hydrated gel. The gel suppresses convective mixing of the fluid phase through which the… …   Dictionary of molecular biology

  • Gel electrophoresis — apparatus – An agarose gel is placed in this buffer filled box and electrical field is applied via the power supply to the rear. The negative terminal is at the far end (black wire), so DNA migrates toward the camera. Classification… …   Wikipedia

  • Polyacrylamide gel — A Polyacrylamide Gel is a separation matrix used in electrophoresis of biomolecules, such as proteins or DNA fragments. Traditional DNA sequencing techniques such as Maxam Gilbert or Sanger methods used polyacrylamide gels to separate DNA… …   Wikipedia

  • Agarose gel electrophoresis — is a method used in biochemistry and molecular biology to separate DNA, or RNA molecules by size. This is achieved by moving negatively charged nucleic acid molecules through an agarose matrix with an electric field (electrophoresis). Shorter… …   Wikipedia

  • sodium dodecyl sulphate polyacrylamide gel electrophoresis — Abbreviation: SDS PAGE A widely employed electrophoretic method for the separation of proteins from biological samples. The sodium dodecyl sulphate gives a uniform charge density to the surface of proteins or nucleic acids, so that their rate of… …   Glossary of Biotechnology

  • Two-dimensional gel electrophoresis — Two dimensional gel electrophoresis, abbreviated as 2 DE or 2 D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels.Basis for… …   Wikipedia

  • electrophoresis — n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet sense 1] Syn: cataphoresis.… …   The Collaborative International Dictionary of English

  • Native Gel Electrophoresis — is a technique used mainly in protein electrophoresis where the proteins are not denatured and therefore separated based on their charge to mass ratio.The two main types of native gels used in protein electrophoresis are polyacrlylamide gels and… …   Wikipedia

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